Comparative analysis of RNA sequencing methods for degraded or low-input samples

 At 2014-03-09 08:36:46 AM  |   209

RNA-seq is an effective method for studying the transcriptome, but it can be difficult to apply to scarce or degraded RNA from fixed clinical samples, rare cell populations or cadavers. Recent studies have proposed several methods for RNA-seq of low-quality and/or low-quantity samples, but the relative merits of these methods have not been systematically analyzed.  Read more

Highly Multiplexed Subcellular RNA Sequencing in Situ

 At 2014-02-27 11:37:08 PM  |   153

Scientists can now take snapshots of where and how thousands of genes are expressed in intact tissue samples, ranging from a slice of a human brain to the embryo of a fly. Read more