Punchline: use spike-ins for every cell in your single-cell experiments to adjust for relative RNA levels per cell.
Single-cell RNA-seq can yield valuable insights about the variability within a population of seemingly homogeneous cells. We developed a quantitative statistical method to distinguish true biological variability from the high levels of technical noise in single-cell experiments. Our approach quantifies the statistical significance of observed cell-to-cell variability in expression strength on a gene-by-gene basis. We validate our approach using two independent data sets from Arabidopsis thaliana and Mus musculus. Read more
Purdue and West Virginia University researchers are the first to sequence the genome of the golden eagle, providing a bird's-eye view of eagle features that could lead to more effective conservation strategies. Read more